The cellular responses to steroids are mediated by 2 general mechanisms: genomic and rapid/nongen... more The cellular responses to steroids are mediated by 2 general mechanisms: genomic and rapid/nongenomic effects. Identification of the mechanisms underlying aldosterone (ALDO)'s rapid vs their genomic actions is difficult to study, and these mechanisms are not clearly understood. Recent data suggest that striatin is a mediator of nongenomic effects of estrogen. We explored the hypothesis that striatin is an intermediary of the rapid/nongenomic effects of ALDO and that striatin serves as a novel link between the actions of the mineralocorticoid and estrogen receptors. In human and mouse endothelial cells, ALDO promoted an increase in phosphorylated extracellular signal-regulated protein kinases 1/2 (pERK) that peaked at 15 minutes. In addition, we found that striatin is a critical intermediary in this process, because reducing striatin levels with small interfering RNA (siRNA) technology prevented the rise in pERK levels. In contrast, reducing striatin did not significantly affect 2 well-characterized genomic responses to ALDO. Down-regulation of striatin with siRNA produced similar effects on estrogen's actions, reducing nongenomic, but not some genomic, actions. ALDO, but not estrogen, increased striatin levels. When endothelial cells were pretreated with ALDO, the rapid/nongenomic response to estrogen on phosphorylated endothelial nitric oxide synthase (peNOS) was enhanced and accelerated significantly. Importantly, pretreatment with estrogen did not enhance…
Cuadernos De Medicina Psicosomatica Y Psiquiatria De Enlace, 2005
Resumen El objetivo del presente estudio ha sido examinar el efecto de la práctica de qigong sobr... more Resumen El objetivo del presente estudio ha sido examinar el efecto de la práctica de qigong sobre varios parámetros hormonales, la presión arterial, síntomas de ansiedad y la calidad subjetiva de sueño. Veinticinco sujetos tomaron parte en el estudio, doce de los ...
The cellular responses to steroids are mediated by 2 general mechanisms: genomic and rapid/nongen... more The cellular responses to steroids are mediated by 2 general mechanisms: genomic and rapid/nongenomic effects. Identification of the mechanisms underlying aldosterone (ALDO)'s rapid vs their genomic actions is difficult to study, and these mechanisms are not clearly understood. Recent data suggest that striatin is a mediator of nongenomic effects of estrogen. We explored the hypothesis that striatin is an intermediary of the rapid/nongenomic effects of ALDO and that striatin serves as a novel link between the actions of the mineralocorticoid and estrogen receptors. In human and mouse endothelial cells, ALDO promoted an increase in phosphorylated extracellular signal-regulated protein kinases 1/2 (pERK) that peaked at 15 minutes. In addition, we found that striatin is a critical intermediary in this process, because reducing striatin levels with small interfering RNA (siRNA) technology prevented the rise in pERK levels. In contrast, reducing striatin did not significantly affect 2 well-characterized genomic responses to ALDO. Down-regulation of striatin with siRNA produced similar effects on estrogen's actions, reducing nongenomic, but not some genomic, actions. ALDO, but not estrogen, increased striatin levels. When endothelial cells were pretreated with ALDO, the rapid/nongenomic response to estrogen on phosphorylated endothelial nitric oxide synthase (peNOS) was enhanced and accelerated significantly. Importantly, pretreatment with estrogen did not enhance…
Cuadernos De Medicina Psicosomatica Y Psiquiatria De Enlace, 2005
Resumen El objetivo del presente estudio ha sido examinar el efecto de la práctica de qigong sobr... more Resumen El objetivo del presente estudio ha sido examinar el efecto de la práctica de qigong sobre varios parámetros hormonales, la presión arterial, síntomas de ansiedad y la calidad subjetiva de sueño. Veinticinco sujetos tomaron parte en el estudio, doce de los ...
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