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DNA barcoding has great potential as a species identification tool because it is practical and affordable to perform and, more often than not, shows species-level separations. Currently, efforts are on to barcode all major groups of animals including insects and millions of species have been barcoded. Other than as a species identification tool, DNA barcoding has been applied to solve many complex ecological phenomena. DNA barcoding is emerging as a simple and very reliable tool for studying host-parasitoid interactions and to establish the host range of phytophagous insects which has changed the perspective of many food webs. DNA barcoding has shown potential as incontestable identification tool for biosecurity purposes with global application. DNA barcoding is also being utilised to document the biodiversity of past and present with reasonable efficiency.
Proceedings of the National Academy of Sciences
Complementary molecular information changes our perception of food web structureHow networks of ecological interactions are structured has a major impact on their functioning. However, accurately resolving both the nodes of the webs and the links between them is fraught with difficulties. We ask whether the new resolution conferred by molecular information changes perceptions of network structure. To probe a network of antagonistic interactions in the High Arctic, we use two complementary sources of molecular data: parasitoid DNA sequenced from the tissues of their hosts and host DNA sequenced from the gut of adult parasitoids. The information added by molecular analysis radically changes the properties of interaction structure. Overall, three times as many interaction types were revealed by combining molecular information from parasitoids and hosts with rearing data, versus rearing data alone. At the species level, our results alter the perceived host specificity of parasitoids, the parasitoid load of host species, and the web-wide role of predators with a cry...
2011 •
2011 •
Molecular Ecology Resources
Molecular detection of trophic links in a complex insect host-parasitoid food web2011 •
Many of the arthropod species that are important pests of agriculture and forestry are impossible to discriminate morphologically throughout all of their life stages. Some cannot be differentiated at any life stage. Over the past decade, DNA barcoding has gained increasing adoption as a tool to both identify known species and to reveal cryptic taxa. Although there has not been a focused effort to develop a barcode library for them, reference sequences are now available for 77% of the 409 species of arthropods documented on major pest databases. Aside from developing the reference library needed to guide specimen identifications, past barcode studies have revealed that a significant fraction of arthropod pests are a complex of allied taxa. Because of their importance as pests and disease vectors impacting global agriculture and forestry, DNA barcode results on these arthropods have significant implications for quarantine detection, regulation, and management. The current review discusses these implications in light of the presence of cryptic species in plant pests exposed by DNA barcoding. Résumé : Plusieurs des espèces d'arthropodes qui constituent d'importants ravageurs en agriculture en foresterie sont impossibles a ` distinguer sur la base morphologique au cours de certains stades de vie. Il est impossible d'en distinguer certains a ` tous les stades. Au cours de la dernière décennie, l'adoption du codage a ` barres de l'ADN s'est accrue tant pour l'identification des espèces connues que cryptiques. Bien qu'il n'y ait pas eu d'effort visant a ` développer une banque de références pour ces espèces, des séquences de référence sont présentement disponibles pour 77 % des 409 espèces d'arthropodes cataloguées parmi les bases de données de ravageurs importants. Outre le développement de la banque de références nécessaire a ` l'identification des spécimens, les études antérieures ont révélé qu'une portion significative des arthropodes nuisibles sont composés d'un ensemble de taxons appar-entés. En raison de leur importance en tant que ravageurs ou vecteurs de maladies ayant un impact global en agriculture et en foresterie, les résultats du codage a ` barres de ces arthropodes ont des implications importantes en matière de détection, de réglementation et de gestion de quarantaines. Dans cette synthèse, les auteurs discutent des implications a ` la lumière de la présence d'espèces cryptiques, au sein de ravageurs de plantes, mises au jour grâce au codage a ` barres de l'ADN. [Traduit par la Rédaction]
2011 •
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Molecular Ecology Resources
DNA barcode analysis of butterfly species from Pakistan points towards regional endemism2013 •
2013 •
Molecular Ecology Resources
DNA barcoding and mini-barcoding as a powerful tool for feather mite studies2015 •
2010 •
Genetics and Molecular Research
ReviewDNA barcode information for the sugar cane moth borer Diatraea saccharalis2008 •
2012 •
Proceedings of the National Academy of Sciences
Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections2008 •
2012 •
Journal of Hymenoptera Research
Hyperparasitoid wasps (Hymenoptera, Trigonalidae) reared from dry forest and rain forest caterpillars of Area de Conservación Guanacaste, Costa Rica2012 •
Biodiversity and Conservation
A critical review on the utility of DNA barcoding in biodiversity conservation2012 •
Molecular Ecology …
Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity2009 •
Proceedings of the National Academy of Sciences
DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae)2011 •
Molecular Ecology Resources
Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens2014 •
Molecular Ecology …
Integration of DNA barcoding into an ongoing inventory of complex tropical …2011 •
2011 •
Proceedings of the Royal Society B: Biological Sciences
Limited performance of DNA barcoding in a diverse community of tropical butterflies2007 •
2010 •
Systematic Entomology
Protein-protein interactions of the cytochrome c oxidase DNA barcoding region2012 •
Biological Control
DNA barcoding of endoparasitoid wasps in the genus Anicetus reveals high levels of host specificity (Hymenoptera: Encyrtidae2011 •
BMC ecology
The diversity and biogeography of the Coleoptera of Churchill: insights from DNA barcoding2013 •
Molecular Ecology
Mitochondrial barcodes are diagnostic of shared refugia but not species in hybridizing oak gallwasps2012 •
Molecular Ecology Resources
Applying DNA barcoding for the study of geographical variation in host-parasitoid interactions2011 •
2012 •
Proceedings of the …
DNA Barcodes Affirm That 16 Species of Apparently Generalist Tropical Parasitoid Flies (Diptera, Tachinidae) Are Not All Generalists2007 •