Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran
- PMID: 21365453
- DOI: 10.1007/s00436-011-2288-5
Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran
Abstract
This study investigated the occurrence of free-living amoebae (FLA) in immunodeficiency wards of hospitals in Tehran, Iran. A total of 70 dust and biofilm samples from wards serving transplant, pediatric (malignancies), HIV, leukemia and oncology patients of five university hospitals were collected and examined for the presence of FLA using culturing and molecular approaches. Based on the morphology of the amoebae in plate cultures, primer sets were applied for molecular identification of Acanthamoeba, vahlkampfiid amoebae and Hartmannella. Out of 70 samples, 37 (52.9%) were positive for FLA. Acanthamoeba belonged to the T4 genotype was the most prevalent isolate. Presence of the T4 genotype on medical instruments, including an oxygen mask in an isolation room of an immunodeficiency pediatric ward, should be of concern for health authorities. Acanthamoeba T5 genotypes, Hartmannella vermiformis, and Vahlkampfia avara were also present. These results highlight a clear need for greater attention to improved disinfection, especially where susceptible patients, such as those who are immune-suppressed, are served. To our knowledge, this is the first report of these FLA in immunodeficiency wards in Iran, and also the first to identify Acanthamoeba T5, Hartmannella, and Vahlkampfia in moist habitats, such as biofilms, in this country.
Similar articles
-
Occurrence and molecular characterization of free-living amoeba species (Acanthamoeba, Hartmannella, and Saccamoeba limax) in various surface water resources of Iran.Parasitol Res. 2015 Dec;114(12):4669-74. doi: 10.1007/s00436-015-4712-8. Epub 2015 Sep 8. Parasitol Res. 2015. PMID: 26346454
-
Thermophilic potentially pathogenic amoebae isolated from natural water bodies in Poland and their molecular characterization.Acta Parasitol. 2014 Sep;59(3):433-41. doi: 10.2478/s11686-014-0266-7. Epub 2014 Aug 15. Acta Parasitol. 2014. PMID: 25119357
-
Ophthalmology hospital wards contamination to pathogenic free living Amoebae in Iran.Acta Parasitol. 2015 Sep;60(3):417-22. doi: 10.1515/ap-2015-0057. Acta Parasitol. 2015. PMID: 26204177
-
Isolation, identification, and phylogenetic analysis of potentially pathogenic free-living amoebae isolated from nasal and oral mucosa of HIV/AIDS patients in Iran.Parasitol Res. 2019 Oct;118(10):3061-3066. doi: 10.1007/s00436-019-06448-x. Epub 2019 Sep 9. Parasitol Res. 2019. PMID: 31502076
-
Current Status of Acanthamoeba in Iran: A Narrative Review Article.Iran J Parasitol. 2015 Apr-Jun;10(2):157-63. Iran J Parasitol. 2015. PMID: 26246812 Free PMC article. Review.
Cited by
-
Vermamoeba vermiformis as the etiological agent in a patient with suspected non-Acanthamoeba keratitis.Parasitol Res. 2024 Sep 10;123(9):323. doi: 10.1007/s00436-024-08347-2. Parasitol Res. 2024. PMID: 39254717
-
Identification and genotyping of Acanthamoeba spp. in the water resources of western Iran.Parasite Epidemiol Control. 2023 May 25;22:e00308. doi: 10.1016/j.parepi.2023.e00308. eCollection 2023 Aug. Parasite Epidemiol Control. 2023. PMID: 37638113 Free PMC article.
-
Genotyping of Acanthamoeba Isolated from Hospital Environments and Thermal Water of Recreational Baths in Markazi Province, Iran.Iran J Parasitol. 2023 Jan-Mar;18(1):38-47. doi: 10.18502/ijpa.v18i1.12378. Iran J Parasitol. 2023. PMID: 37197073 Free PMC article.
-
Molecular detection and genotype identification of Acanthamoeba species from bronchoalveolar lavage of patients with pulmonary symptoms suspected of cancer.J Parasit Dis. 2022 Dec;46(4):1028-1035. doi: 10.1007/s12639-022-01524-z. Epub 2022 Aug 18. J Parasit Dis. 2022. PMID: 36457768 Free PMC article.
-
Can Free Living Acanthamoeba Act as a Trojan Horse for SARS-Cov-2 on Viral Survival and Transmission in the Environment? A Narrative Review.Iran J Parasitol. 2022 Apr-Jun;17(2):138-144. doi: 10.18502/ijpa.v17i2.9529. Iran J Parasitol. 2022. PMID: 36032747 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions