Folia Parasitologica 52[4] 295-303 (2005) | DOI: 10.14411/fp.2005.040

Fish-isolated strains of Hartmannella vermiformis Page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions

Iva Dykov�1,2, Zuzana Pindov�2, Ivan Fiala1,2, Helena Dvo��kov�1, Blanka Mach��kov�1
1 Institute of Parasitology, Academy of Sciences of the Czech Republic, Brani�ovsk� 31, 370 05 �esk� Bud�jovice, Czech Republic
2 Faculty of Biological Sciences, University of South Bohemia, Brani�ovsk� 31, 370 05 �esk� Bud�jovice, Czech Republic

Based on morphological and molecular characterisation, four amoeba strains isolated from organs of freshwater fish were identified as Hartmannella vermiformis Page, 1967. Small subunit rRNA gene sequences of these strains expand the set of corresponding complete and almost complete sequences of this species to twelve. A new species-specific oligonucleotide probe inferred from recently available SSU rRNA gene sequences was designed and successfully tested in tissue lesions produced by one strain of H. vermiformis in experimentally infected fish.

Keywords: amphizoic amoebae, Hartmannella vermiformis, morphology, phylogeny, in situ hybridisation

Received: April 14, 2005; Accepted: July 15, 2005; Published: September 1, 2005  Show citation

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Dykov�, I., Pindov�, Z., Fiala, I., Dvo��kov�, H., & Mach��kov�, B. (2005). Fish-isolated strains of Hartmannella vermiformis Page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions. Folia Parasitologica,�52(4),�295-303. doi:�10.14411/fp.2005.040
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